We’ve compiled a list of kit that you may want to include when looking for evidence of big cats. However, you don’t need to have everything on this list—the most important tool in any investigation is your awareness of your surroundings. Pay attention to the details: are the birds giving an alarm call, signaling the presence of a predator? Can you identify the wildlife trails that big cats might also be using? Look for subtle clues, such as hair snagged on wire or branches, and pugmarks in soft ground.
Above all, the ability to observe, record, and measure what you find is the most crucial part of an investigation. Noting where and how you discovered potential evidence can provide valuable insights and help build a clearer picture of these elusive animals.
Once you have found evidence, you need to collect samples, for analysis. Were are very grateful to Professor Robin Allaby, of Warwickshire University, for putting together instructions for us, on how to collect big cat samples.
Be sure to read the guide below on how to collect samples for laboratory analysis.
When investigating potential big cat evidence, it is essential to respect the environment and local laws. Do not disturb or interfere with wildlife, their habitats, or any plants you encounter. Always seek permission before entering private land, and never trespass or damage property.
Responsible investigation ensures that both the natural world and the reputation of the big cat research community remain protected.
Instructions provided by Professor Robin Allaby.
The types of sample that might be taken include a) a swab from a predated carcass, b) a hair (or preferably hairs plural) snagged on an object such as a fence post or c) scat.
We supply swab kits which consist of a long tube, cotton bud stick and stabilizing buffer. Given that swabbing must be done within the first few hours (preferably less than 12) after a kill for detection to be successful, one must have swab kits BEFORE carcasses are discovered. These can be obtained through contacting Ecowarwicker (bats@warwick.ac.uk). We recommend repeated swabbing around clear bite areas. Swab samples can then be sent by post in a jiffy bag to Ecowarwicker (see address at Exotic species test | Ecowarwicker Ecology ) We recommend storage at 4˚C (fridge temperature) until posting.
It is true that DNA is trapped right through the length of a hair shaft, although the older the section of hair shaft, the more damaged the DNA will be. The most ideal hair sample is one in which the root hair cell at the base of the shaft is included – the ‘living’ part of the hair. For this type of sample as many hairs as possible is best. A single hair can possibly work, but the levels of DNA are very low, especially if no root is included. These can be placed in a plastic tube or bag and sent to Ecowarwicker.
It is difficult to identify species by scat accurately by sight. However, if there is a scat that is sufficiently suspicious it is relatively easy to obtain the defaecator’s DNA. A small amount of such a sample (1cm3 is sufficient) can be placed in a plastic tube and sent to Ecowarwicker in a jiffy bag. We caution that we have identified a large number of foxes this way.
Tooth pits, or tooth marks, are a forensic technique used to identify the presence of big cats in an area by analysing the remains of their prey animals. Tooth pit analysis involves examining the bones of prey animals for characteristics such as tooth pits, scores, punctures, or furrowing. The process includes using dental putty to create casts of the bones, which are then measured with digital callipers to determine the length and spacing of the tooth pits. These measurements are compared to the scale and pattern of known carnivores to identify the predator.
This is the triangle pattern to look for on bones of suspected prey, especially on mandibles and pelvic bones. The result is notches or 'pits' made by the sharp cusps of the carnassial, and is a distinctive dimension to cats.
Fig. 1:
The cusps of an upper carnassial tooth of a puma.
Fig 2:
An example of the triangle pattern on a young elk pelvic bone in Colorado.
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